Shameik Moore as Miles Morales / Spider-Man
Hailee Steinfeld as Gwen Stacy / Spider-Woman
Brian Tyree Henry as Jeff Morales
Lauren Vélez as Rio Morales
Jake Johnson as Peter B. Parker / Spider-Man
Jason Schwartzman as Johnathon Ohnn / the Spot
Issa Rae as Jess Drew / Spider-Woman
Karan Soni as Pavitr Prabhakar / Spider-Man India
Daniel Kaluuya as Hobie Brown / Spider-Punk
Oscar Isaac as Miguel O'Hara / Spider-Man 2099